Journal: Analytical and Bioanalytical Chemistry

Article Title: Procedure providing SI-traceable results for the calibration of protein standards by sulfur determination and its application on tau

doi: 10.1007/s00216-022-03974-z

Figure Lengend Snippet: Recoveries of sulfur-containing compounds in the filtrate after membrane filtration using different mobile phases. Uncertainties: standard deviation of replicate measurements ( N = 3). BSA: NIST SRM 927e, Met: methionine

Article Snippet: The protein quantification method was developed and tested on the certified reference material SRM 927e bovine serum albumin (BSA) from the National Institute of Standards and Technology (NIST), as well as commercially available avidin.

Techniques: Membrane, Filtration, Standard Deviation, Avidin-Biotin Assay

Journal: Analytical and Bioanalytical Chemistry

Article Title: Procedure providing SI-traceable results for the calibration of protein standards by sulfur determination and its application on tau

doi: 10.1007/s00216-022-03974-z

Figure Lengend Snippet: Fractions of non-protein-bound sulfur determined in BSA (NIST SRM 927e) and avidin after separation by membrane filtration. Uncertainty ( U ): combined uncertainty of the standard deviation and the uncertainties of each measurement ( N = 3), given as expanded uncertainty ( k = 2)

Article Snippet: The protein quantification method was developed and tested on the certified reference material SRM 927e bovine serum albumin (BSA) from the National Institute of Standards and Technology (NIST), as well as commercially available avidin.

Techniques: Avidin-Biotin Assay, Membrane, Filtration, Standard Deviation

Journal: Analytical and Bioanalytical Chemistry

Article Title: Procedure providing SI-traceable results for the calibration of protein standards by sulfur determination and its application on tau

doi: 10.1007/s00216-022-03974-z

Figure Lengend Snippet: Sulfur and protein mass fraction in BSA (NIST SRM 927e) stock solution and lyophilized avidin powder. Results with expanded uncertainties U with k = 2. BSA: 4 separate sample preparations, avidin: 3 separate sample preparations

Article Snippet: The protein quantification method was developed and tested on the certified reference material SRM 927e bovine serum albumin (BSA) from the National Institute of Standards and Technology (NIST), as well as commercially available avidin.

Techniques: Avidin-Biotin Assay

Journal: Analytical and Bioanalytical Chemistry

Article Title: Procedure providing SI-traceable results for the calibration of protein standards by sulfur determination and its application on tau

doi: 10.1007/s00216-022-03974-z

Figure Lengend Snippet: Comparison of protein quantification methods applied to certified reference materials BSA 927e and 927d

Article Snippet: The protein quantification method was developed and tested on the certified reference material SRM 927e bovine serum albumin (BSA) from the National Institute of Standards and Technology (NIST), as well as commercially available avidin.

Techniques: Comparison, Concentration Assay, Functional Assay

Journal: Journal of Analytical Methods in Chemistry

Article Title: Development of a Univariate Membrane-Based Mid-Infrared Method for Protein Quantitation and Total Lipid Content Analysis of Biological Samples

doi: 10.1155/2014/657079

Figure Lengend Snippet: Protein calibration curve developed using NIST BSA diluted into PBS. A series of ten dilutions (in triplicate), spanning the range 0.125–5 mg/mL, was used to prepare a calibration curve. Amide I signal strength delivered by each concentration point was fitted to a regression line.

Article Snippet: For all reported experiments, the system was calibrated using bovine serum albumin (BSA) from the National Institute of Standards and Technology (NIST) diluted in phosphate-buffered saline (PBS).

Techniques: Concentration Assay